Analytical Standard Arachidonic Acid Ethyl Ester CAS 506-32-1 99% Purity Liquid

Arachidonic acid (CAS 506-32-1) is normally quantified as its fatty acid methyl or ethyl ester, because the free acid tails badly on a GC column. This standard supplies the ethyl ester at 99 percent purity, prepared from fermentation derived ARA and purified by preparative chromatography, so that laboratories can calibrate omega-6 methods without the uncertainty of a technical grade oil.

The material is characterised by GC with flame ionisation detection and confirmed by GC-MS, with the purity, retention index and the level of neighbouring fatty acid esters reported on the certificate. It is filled under nitrogen into amber glass and shipped in foam lined cartons, because a standard that has oxidised in transit is worse than no standard at all.

• Purity 99% by GC: a clean calibration point for quantitative omega-6 methods
• Ethyl ester form: sharp peaks and no tailing on polar and non polar GC columns
• Identity confirmed by GC-MS: verification that the main peak is the ARA ethyl ester and not a positional isomer
• Nitrogen filled amber packaging: slows oxidation of the four double bonds during storage and use
• Neighbouring esters quantified: EPA, DHA, linoleic and oleic ester levels reported so interference can be assessed
• Documentation for method files: chromatogram, COA and retention index supplied with every ampoule

Used to calibrate GC-FID and GC-MS methods for fatty acid profiling of infant formula, supplements and biological samples, as an internal standard in lipid quantification, and as a reference for identity testing of ARA containing ingredients.

A working solution is normally prepared at 1 to 5 mg/mL in hexane or isooctane and stored at -18 degrees C in a sealed vial for up to one month. Our analytical note gives a suggested oven programme on a 60 m biscyanopropyl column and the expected retention times relative to the C20 to C24 ester series.

Packed in 100 mL and 500 mL amber glass bottles with PTFE lined caps, nitrogen filled and foam packed in cartons for air freight. Each container carries the lot number, purity, packaging date and storage instruction.

Store at -18 degrees C under nitrogen, protected from light; allow the container to reach room temperature before opening to avoid condensation. Shipments leave within 7 to 15 working days with COA and chromatogram attached.

Every lot ships with a Certificate of Analysis including the GC chromatogram, the calculated purity, the identified neighbouring ester peaks and the GC-MS confirmation report. A retention sample is stored at -18 degrees C for 36 months.

For accredited laboratories we can provide a statement of traceability and the measurement uncertainty associated with the assigned purity. Custom blends of omega-3 and omega-6 esters for multi analyte calibration can be prepared on request.