Peptide Synthesis Grade HFIP CAS 920-66-1 Cleavage Reagent and SPPS Solvent

In solid phase peptide synthesis, HFIP has two jobs. It is a cleavage and deprotection additive in the cocktail, and it is a solvent for aggregating sequences where the growing chain folds back on itself and stops reacting. Both uses are sensitive to water and to acid content, because the cocktail is already a strong acid mixture and a wet solvent changes the balance.

This grade is 99.9% with water at 100 ppm and free fluoride under 5 ppm, packed under argon. It is the specification we send to peptide houses, and the batch file carries the water, fluoride and residue figures plus the chromatogram, which is generally what a process group needs for its own release testing.

• Water 100 ppm maximum: no drift in cleavage cocktail strength between preparations
• Free fluoride under 5 ppm: no side reactions from the solvent in an acid sensitive sequence
• Residue under 20 ppm: no impurity peaks that could be mistaken for a peptide fragment
• Argon blanketed bottles: stable through a campaign rather than a single run
• Batch chromatogram: solvent purity separated from peptide purity in troubleshooting
• Scale from 1 kg to 25 kg: development and GMP adjacent supply on the same specification

Cleavage cocktails in SPPS at 10 to 30% of the mixture, dissolution of hydrophobic protected fragments, and coupling steps for sequences that aggregate in DMF or NMP.

Prepare the cocktail fresh and keep it covered; HFIP evaporates quickly at room temperature and the composition drifts if the vessel sits open. For aggregation problems, a 20 to 30% HFIP addition in DMF is a common starting point before changing the resin or the coupling reagent.

1 kg fluorinated bottle and 5 kg can, argon filled, plus 25 kg drum for production campaigns. Labels record the water and fluoride figures at release.

Store between 15 and 25 degrees C, sealed under argon. Air freight for bottles and cans, sea freight for drums from Shanghai.

COA with assay, water, free fluoride, residue, colour and density, plus the GC trace. A retention sample per lot is kept for 24 months.

SDS and a residual solvent statement are available, and we can add a customer specific test such as chloride by ion chromatography when a method requires it.